Development of an innovative maceration technique to optimize extraction and phase partition of natural products

The active component extraction from plants is the first crucial step in natural product research. For non-targeted with an objective to isolate and characterize as many compounds possible, most classical technique, simplest implement, Soxhlet extraction; however, it does not allow retrieving all plant (when additionally cause artifacts during long heating process). second used technique by successive macerations using solvents of increasing polarity. While this method frequently used, few studies are available rationalize optimize it. Furthermore, requires some enhancement mainly for efficiency, environmental time constraint reasons. Here, we present innovative a mixture aim simultaneously improving yield, partition between different phases reducing volume solvents. Triphasic systems were prepared mixing five (n-heptane, ethyl acetate, acetonitrile, butan-1-ol, water) various proportions. To validate method, efficient triphasic system was subsequently perform three polarity gradient on model before being extended several alpine plants. Our results showed overall good yield compared conventional maceration techniques, while phase